Development of novel methods for the toxicity assessment of multi-component chemical mixtures to humans and the ecosystem

Program at a glance

Funding Agencies

Cyprus Research Promotion Foundation (DESMI 2009-2010), Republic of Cyprus, European Regional Development Fund

Program Period


Project Acronym


Project Code


Project Title

Development of novel methods for the toxicity assessment of multi-component chemical mixtures to humans and the ecosystem

Project Coordinator

Prof. Despo Fatta Kassinos, Department of Civil and Environmental Engineering, and Nireas-International Water Research Center, University of Cyprus, Cyprus


Laboratory of Department of Life and Health Sciences, University of Nicosia

Medical Research Center, Faculty for Clinical Medicine Mannheim, Ruprecht-Karls-University Heidelberg

Total Budget

69,936 €

Project Website

Program Overview

The release of active pharmaceutical ingredients to the environment is an issue of increasing importance which takes place for many years now. This happens through the Urban Wastewater Treatment Plants effluents’ discharges in which multi-component mixtures (parent compounds, metabolites and transformation products) of active pharmaceutical ingredients exist. The number of studies dealing with the effects of active pharmaceutical ingredients residues on aquatic organisms and risk assessment is still limited and scarce, particularly regarding long-term studies and only very few studies have observed the effects of mixtures.

The TOMIXX research project aimed at: (1) leveraging and integrating existing expertise currently distributed among partners, thus leading to an upgraded profile for them in the field of multi-component impact assessment and to new relevant scientific insights in the field of environmental science, (2) transfering specialized knowledge and promoting lasting professional cooperation between the participant organisations, governmental and private sector in the field of xenobiotics’ impact assessment and water resources quality protection, (3) promoting excellence in the research field of advanced water management and developing and/or enhance local expertise in the field of xenobiotics in water and wastewater, (4) evaluating the potential impacts of active pharmaceutical ingredients and their multi-component mixtures to humans and the ecosystem, which is a new field of scientific research attracting intense worldwide interest, (5) contributing to the implementation of the Water Framework Directive since the project will provide new insights for developing emission limit values and environmental quality standards with respect to active pharmaceutical ingredients currently absent from the priority lists circulated; and (6) enhancing the acceptance and practices related to wastewater reuse by determining the most relevant issues relating to reuse and adverse effects.

Working Packages

WP1: Project Management
WP2: Dissemination and Exploitation of Results
WP3: Investigating the state-of-the-art regarding research on toxicity of multi-component mixtures
WP4: Acute and chronic ecotoxicity assessment of active pharmaceutical ingredients and their photo-transformation products to aquatic organisms
WP5: Ecotoxicity assessment of multi-component chemical mixtures to aquatic organisms
WP6: Mutagenicity assessment of multi-component chemical mixtures to human cells
WP7: Development of a novel screening assay for endocrine and cytotoxicity assessment to human cells
WP8: Development of novel omics techniques to assess toxicity of multi-component chemical mixtures to human

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